Template - rDNA formatted table
Guidance for completing this table:
You must first save this document to your computer and then use the Attachment Section within the eIBC system to upload your table to the registration document.
Columns within the spreadsheet include:
- Nucleic Acid Product or Insert: identify the gene or sequence that is being cloned or expressed from the vector; examples include GFP, gRNA for PPARg2, Cas9.
- Biological Source: list the species from which the gene was cloned or the sequence upon which the insert was based; examples include A. Victoria, human/mouse, S. pyogenes.
- Some gRNAs, miRNAs, etc. may be synthetically made; however, the committee is looking for the species on which you have based the sequence of these inserts.
- Function: list the function of the insert or the function of the gene which you are targeting; examples include fluorescent protein, transcription factor that regulates adipogenesis; nuclease.
- Target Vector: list the plasmids and/or viral vectors that you will be using to clone or express your nucleic acid product or insert; examples include AAV, pcDNA3.1, pSUPER.
- Many labs use multiple cloning or expression plasmids and can list these in a single entry, an example includes expression plasmids such as pcDNA3.1.
- When retroviral or lentivirual vectors are used, specify the virus upon which the vector is based; examples include MLV, FIV, HIV, etc.
- Host: list the cell line(s) and/or biological organisms that will receive your rDNA construct; examples include human and mouse cell lines, E.coli cloning strains, S. aureus-MRSA, mouse.
- Listing the species from which the cell line was obtained is required as this allows the committee and Biosafety staff to identify training requirements and proper handling conditions.
- BSL*: list the appropriate biosafety level for handling the vector and/or the host; examples include BSL2 for human cell lines and S.aureus-MRSA, BSL1 for mouse cell lines and E.coli cloning strains.
- The IBC has determined that all viral vectors should be handled at BSL2.
- ABSL*: list the appropriate animal biosafety level for handling the animal following administration of the recombinant material; examples include ABSL1 for replication defective AAV or expression plasmids, ABSL2c for replication defective Adenovirus, ABSL2a for S. aureus-MRSA.
*- The BSL and ABSL will be reviewed by the IBC and any concerns or changes to the submitted containment levels will be communicated to research staff.
Any questions regarding the rDNA formatted table or the eIBC Management system can be directed to Biosafety Staff at ehs-rdna@uiowa.edu or Nyree Mortensen, 319-353-5679