As addressed by the NIH Guidelines for Research Involving Recombinant DNA Molecules (NIH Guidelines), particular research with E. coli K-12 derivatives is exempt from the requirements of the NIH Guidelines.
Ancestral E. coli K-12
Strain Designation |
Origin or Collection |
| 58 |
Stanford Strain |
| 679 | Stanford strain |
| WG1 | Wisconsin strain |
E. coli K-12 Derivatives
| Strain Designation | Origin or Collection | Strain Designation | Origin or Collection |
|---|---|---|---|
| 5K | Lab strain | JM83 | Lab strain |
| 58-161 | Lab strain | JM101 | Lab strain |
| AB284 | Lab strain | MB408 | Lab strain |
| AB311 | Lab strain | P678 | Lab strain |
| AG1 | Lab strain | PA 309 | Lab strain |
| C600 | Lab strain | SURE | Lab strain |
| Cavalli Hfr | Lab strain | TG1 | Lab strain |
| DH1 | Lab strain | W208 | Lab strain |
| Dh5a | Lab strain | W945 | Lab strain |
| DP50 | Lab strain | W1485 | Lab strain |
| EMG2 | Lab strain | W3110 | Lab strain |
| H1443 | Lab strain | WA704 | Lab strain |
| HB101 | Lab strain | XL1-Blue | Stratagene |
| Hfr3000 | Lab strain | XLOLR | Stratagene |
| Hfr 3000 X74 | Lab strain | Y10 | Lab Strain |
| HMS 174 | Novegen | YN2980 | Lab Strain |
| JC9387 | Lab strain |
For additional strains and information regarding K-12 derivatives please go to the following website:
http://cgsc.biology.yale.edu/
NOTE: E. coli Laboratory Strains NOT Derived from K-12
The following E. coli laboratory strains are not derived from K-12 and therefore any research utilizing these strains is not exempt from the NIH Guidelines.
| Strain Designation | Origin or Collection |
|---|---|
| B | Laboratory strain |
| B-3 | Laboratory strain |
| BL21 | Novagen |
| B/R | Laboratory strain |
| C | Laboratory strain |
| TOPP | Stratagene |
Reference
Kuhnert, P., J. Nicolet, J. Frey. 1995. Rapid and accurate identification of Escherichia coli K-12 strains. Applied and Environmental Microbiology. 61:4135-4139.