BSL2 / RG2 Agent or “Large Spill”
A spill is considered to be large if it is difficult to contain within the laboratory or the facility, and/or it constitutes a significant health hazard. This type of spill may require special assistance in controlling and clean up.
- Evacuate the area immediately.
- If biological safety cabinet or fume hood is in the room, leave it on and immediately exit the room. Close and lock the door.
- Post a "Biohazard" and "Do Not Enter" sign on the door to keep people out of the area and to prevent the spread of the contaminant.
- Notify supervisor and EHS's Biological Safety Section.
- Thoroughly wash face, hands and any exposed body area. Remove all contaminated clothing, and decontaminate (autoclave, if necessary). If necessary, use an emergency shower in the immediate area.
- Allow at least 30 minutes for droplets to settle and aerosols to be reduced before reentering.
- Don protective equipment (long sleeved lab coat, disposable gloves, safety goggles and face shield and disposable shoe covers, if needed).
- If the spill is large, apply absorbent booms or dike around the spill area to avoid spreading.
- Decontaminate with an appropriate disinfectant. Pour the disinfectant slowly around the spill, not on the spill, to avoid aerosolizing the material.
- Cover spill area with paper towels or absorbent pads soaked in disinfectant.
- Allow a 20 minute disinfectant contact period.
- Wipe down all surfaces that may have been splashed.
- Clean up the liquid working from the outside of the spill area inward to avoid spreading the spill.
- Using an autoclavable dust pan and squeegee, transfer all contaminated glassware or sharp material into a sharp's bucket.
- Re-wipe spill area with disinfectant.
- Dispose of all cleanup items in the proper container and autoclave.
- Wash hands and any exposed skin with soap and water.
- For large spills, contact EHS's Biological Safety Section for assistance in determining when additional decontamination is necessary.