8.7.1 Autoclaving

Autoclaving is the most effective and reliable means of sterilizing laboratory materials.  Autoclaving sterilizes material using saturated steam under pressure.  Standard autoclave cycles for commonly used material are shown below:

  • Laundry: 121°C for 30 minutes with 15 minutes prevacuum of 27 inches of mercury (in. Hg)
  • Glassware and trash: 121°C for 1 hour with 15 minutes prevacuum of 27 in. Hg
  • Liquids: 121°C for 1 hour for each gallon