7.2.3 Safety Equipment (in addition to those listed for ABSL1)

  • Properly maintained biological safety cabinets, personal protective equipment (e.g., gloves, lab coats, face shields, respirators, etc.) and/or other physical containment devices or equipment, are used whenever conducting procedures with a potential for creating aerosols or splashes.  These include necropsy of infected animals, harvesting of tissues or fluids from infected animals or eggs, and intranasal inoculation of animals. 
  • When indicated by risk assessment, animals are housed in primary biosafety containment equipment appropriate for the animal species, such as solid wall and bottom cages covered with filter bonnets for rodents, or larger cages placed in inward flow ventilated enclosures or other equivalent primary containment systems for larger animal cages. 
  • A risk assessment should determine the appropriate type of personal protective equipment to be utilized.  Scrub suits and uniforms are removed before leaving the animal facility.  Reusable clothing is appropriately contained and decontaminated before being laundered.  Laboratory and protective clothing should never be taken home.
  • Gowns, uniforms, laboratory coats, and personal protective equipment are worn while in the areas where infectious materials and/or animals are housed or manipulated and removed prior to exiting.  Disposable personal protective equipment and other contaminated waste are appropriately contained and decontaminated prior to disposal.
  • Eye and face protection (goggles, mask, face shield or other splatter guard) are used for anticipated splashes/sprays from infectious or other hazardous materials when the animal or microorganisms must be handled outside the BSC or containment device.  Eye and face protection must be disposed of with other contaminated laboratory waste or decontaminated before reuse.  Persons who wear contact lenses should also wear eye protection when entering areas with potentially high concentrations or airborne particulates.
  • Persons having contact with non-human primates should assess risk of mucous membrane exposure and wear appropriate protective equipment (e.g., masks, goggles, face shields, etc.) as needed.  Respiratory protection is worn based upon risk assessment.