6.3.3 Safety Equipment

  • All procedures involving the manipulation of infectious materials must be conducted within a BSC (preferably Class II or Class III), or other physical containment devices. 
  • Protective lab clothing with a solid-front such as tie-back or wrap-around gowns, scrub suits, or coveralls are worn by workers when in the laboratory.  Protective clothing is not worn outside of the laboratory.  Reusable clothing is decontaminated with appropriate disinfectant before being laundered.  Clothing is changed when contaminated. 
  • Eye and face protection (goggles, mask, face shield or other splatter guard) is used for anticipated splashes or sprays of infectious or other hazardous materials.  Eye and face protection must be disposed of with other contaminated laboratory waste or decontaminated before reuse.  Persons who wear contact lenses in laboratories must also wear eye protection. 
  • Gloves must be worn to protect hands from exposure to hazardous materials. Glove selection should be based on an appropriate risk assessment.  Alternatives to latex gloves should be available.  Gloves must not be worn outside the laboratory.  In addition, BSL-3 laboratory workers should:
  1. Change gloves when contaminated, integrity has been compromised, or when otherwise necessary.  Wear two pairs of gloves when appropriate.
  2. Remove gloves and wash hands when work with hazardous materials has been completed and before leaving the laboratory.
  3. Do not wash or reuse disposable gloves.  Dispose of used gloves with other contaminated laboratory waste.  Hand washing protocols must be rigorously followed.
  • Eye, face and respiratory protection must be used in rooms containing infected animals.