6.3.2 Special Practices (in addition to those listed for BSL2)

  • The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with BSL-3 agents.
  • All procedures involving the manipulation of infectious materials must be conducted within a BSC, or other physical containment devices.  No work with open vessels is conducted on the bench.  When a procedure cannot be performed within a BSC, a combination of personal protective equipment and other containment devices, such as a centrifuge safety cup or sealed rotor, must be used.