6.2.4 Laboratory Facilities (in addition to the requirements for BSL1)

  • Laboratory doors should be self-closing and have locks in accordance with the institutional policies.
  • Laboratories must have a sink for hand washing.  The sink may be manually, hands-free, or automatically operated.  It should be located near the exit door.
  • The laboratory should be designed so that it can be easily cleaned and decontaminated. Carpets and rugs in laboratories are not permitted.
  • Laboratory windows that open to the exterior are not recommended.  However, if a laboratory does have windows that open to the exterior, they must be fitted with screens.
  • BSCs must be installed so that fluctuations of the room air supply and exhaust do not interfere with proper operations. BSCs should be located away from doors, windows that can be opened, heavily traveled laboratory areas, and other possible airflow disruptions.
  • Vacuum lines should be protected with High Efficiency Particulate Air (HEPA) filters, or their equivalent.  Filters must be replaced as needed.  Liquid disinfectant traps may be required.
  • An eyewash station must be readily available. 
  • There are no specific requirements on ventilation systems. However, planning of new facilities should consider mechanical ventilation systems that provide an inward flow of air without recirculation to spaces outside of the laboratory.
  • HEPA filtered exhaust air from a Class II BSC can be safely re-circulated back into the laboratory environment if the cabinet is tested and certified at least annually and operated according to manufacturer’s recommendations.  BSCs can also be connected to the laboratory exhaust system by either a thimble (canopy) connection or a direct (hard) connection.  Provisions to assure proper safety cabinet performance and air system operation must be verified.
  • A method for decontaminating all laboratory wastes should be available in the facility (e.g. autoclave, chemical disinfection, incineration, or other validated decontamination method).