6.2.3 Safety Equipment (in addition to those listed for BSL1)

  • Properly maintained biological safety cabinets, preferably Class II, or other appropriate personal protective equipment or physical containment devices must be used whenever: 
  1. Procedures with a potential for creating infectious aerosols or splashes are conducted.  These may include pipetting, centrifuging, grinding, blending, shaking, mixing, sonicating, opening containers of infectious materials, inoculating animals intranasally and harvesting infected tissues from animals or eggs. 
  2. High concentrations or large volumes of infectious agents are used.  Such materials may be centrifuged in the open laboratory using sealed rotor heads or centrifuge safety cups. 
  • Protective laboratory coats, gowns, smocks or uniforms designated for laboratory use must be worn while working with hazardous materials.  Remove protective clothing before leaving for non-laboratory areas (e.g., cafeteria, library, administrative offices).  Dispose of protective clothing in the laboratory or deposit it for laundering by the University; protective clothing is not to be taken home by personnel.
  • Eye and face protection (goggles, mask, face shield or other splatter guard) must be used for anticipated splashes or sprays of infectious or other hazardous materials when the microorganisms must be manipulated outside the BSC or containment device.  Eye and face protection must be disposed of with other contaminated laboratory waste or decontaminated before reuse.  Persons who wear contact lenses in laboratories should also wear eye protection. 
  • Gloves must be worn to protect hands from exposure to hazardous materials. Glove selection should be based on an appropriate risk assessment.  Alternatives to latex gloves should be available.  Gloves must not be worn outside the laboratory.  In addition, BSL-2 laboratory workers should:
  1. Change gloves when contaminated, integrity has been compromised, or when otherwise necessary.  Wear two pairs of gloves when appropriate.
  2. Remove gloves and wash hands when work with hazardous materials has been completed and before leaving the laboratory.
  3. Do not wash or reuse disposable gloves.  Dispose of used gloves with other contaminated laboratory waste.  Hand washing protocols must be rigorously followed.
  • Eye, face and respiratory protection should be used in rooms containing infected animals as determined by the risk assessment.