6.2.2 Special Practices

  • All persons entering the laboratory must be advised of the potential hazards and meet specific entry/exit requirements.
  • Laboratory personnel must be provided medical surveillance and offered appropriate immunizations for agents handled or potentially present in the laboratory.
  • Each institution must establish policies and procedures describing the collection and storage of serum samples from at-risk personnel.
  • A laboratory-specific biosafety manual must be prepared and adopted as policy.  The biosafety manual must be available and accessible.
  • The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with BSL-2 agents.
  • Potentially infectious materials must be placed in a durable, leak proof container during collection, handling, processing, storage, or transport within a facility.
  • Laboratory equipment should be routinely decontaminated, as well as, after spills, splashes, or other potential contamination.
  1. Spills involving infectious materials must be contained, decontaminated, and cleaned up by staff properly trained and equipped to work with infectious material.
  2. Equipment must be decontaminated before repair, maintenance, or removal from the laboratory.
  • Incidents that may result in exposure to infectious materials must be immediately evaluated and treated according to procedures described in the laboratory biosafety safety manual.  All such incidents must be reported to the laboratory supervisor. Medical evaluation, surveillance, and treatment should be provided and appropriate records maintained.
  • Animals and plants not associated with the work being performed must not be permitted in the laboratory.
  • All procedures involving the manipulation of infectious materials that may generate an aerosol must be conducted within a BSC or other physical containment devices.