5.3 Good Microbiological Techniques (GMT)

​In addition to preventing general accidents, laboratory workers must also be aware of the characteristics and potential hazards of the microorganisms in the laboratory.  GMT can describe a range of techniques and procedures that when applied in a laboratory setting will prevent the contamination of (1) the laboratory workers and the environment and (2) the experiment.  GMT includes:

  • using proper aseptic techniques;
  • wearing appropriate personal protective equipment; 
  • minimizing the production of aerosols or working in a biological safety cabinet when aerosol production is unavoidable; 
  • employing universal precautions with bloodborne pathogens;
  • routinely disinfecting work surfaces and equipment/supplies used in the experiment;
  • immediately cleaning spills according to laboratory protocols;
  • restricting work with infectious material to one area of the laboratory;
  • properly disposing of waste;
  • routinely washing hands following any experiment and after removing gloves; and
  • reporting all accidents/incidents and seeking medical attention if necessary.
Further details regarding these techniques and protocols will be discussed throughout this manual.  GMT should be applied when working with any microorganism, regardless of the biosafety level.