5.11 Sulfur-35 Volatility Control Requirements

Users of volatile S-35 labeled compounds such as methionine and cysteine and high specific-activity thio-labeled nucleotides are required to take steps to control contamination due to the volatility of these compounds.  EHS evaluates requests to use these compounds on a case-by-case basis.

Generally, use of volatile S-35 compounds require the use of activated charcoal cartridges or filter papers during incubation procedures; secondary containment during storage; and venting with a charcoal trap when withdrawing activity from stock vials.  Lab personnel are encouraged to perform contamination surveys following each use of these compounds.